Public Relations
Press Release
April 22, 2010
International Health Management Associates, Inc (IHMA) announces the addition of a new molecular laboratory to its existing microbiology laboratory – Laboratories International for Microbiology Studies. The new addition gives IHMA the capabilities to perform numerous molecular studies on past, present and future bacteria including:
| Organisms | Mechanisms of resistance/Typing | Methods |
| Enterobacteriaceae | ESBLs | DNA micro-array (Check-Points) |
| Characterization of SHV, TEM and CTX-M | PCR-sequencing | |
| AmpC β-lactamases | Multiplex PCR or DNA micro-array (Check-Points) | |
| Detection and characterization of AmpC genes | PCR-sequencing | |
| (MOX, CMY, LAT, ACC, MIR, ACT, FOX..) | ||
| Carbapenemases | Multiplex PCR or DNA micro-array (Check-Points) | |
| Detection and characterization of KPC, IMP, VIM... | PCR-sequencing | |
| Loss of outer membran protein (OMP) | SDS-PAGE | |
| Enterobacteriaceae | Quinolone resistance: | |
| Gram positive cocci | Mutations in DNA gyrase and topoisomerase IV | PCR-sequencing |
| gyrA, gyrB and parC, parE | ||
| Enterobacteriaceae | Plasmid-mediated quinolone resistance | Multiplex PCR, conjugation experiments, plasmid mapping |
| Streptococcus pneumoniae | Serotyping | Multiplex-PCR |
| Streptococci | MLSb resistance | |
| erm, mefs genes | Multiplex-PCR | |
| ribosomal mutations (L4, L22) | PCR-sequencing | |
| 23S rRNA mutations | PCR-RFLP | |
| Staphylococci | mecA, SSCmec types | Multiplex-PCR |
| mupA | ||
| PVL | ||
| Enterobacteriaceae | Molecular typing | RAPD |
| Staphylococci | ERIC-PCR | |
| PFGE |
The laboratory will continue to be under the direction of by Daryl J. Hoban, PhD, FCCM, D(ABMM), F(AAM) and managed by Associated Director, Meredith Hackel, PhD, MPH. We are please to announce the addition of Christine Lascols (Master Science in Molecular Microbiology) as the Lead Molecular Microbiologist for the new laboratory addition.